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Test Code TEMZS Temazepam and Oxazepam, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Assessing compliance

 

Monitoring for appropriate therapeutic level

 

Assessing temazepam toxicity

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Temazepam and Oxazepam, S

Specimen Type

Serum Red

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Clinical Information

Temazepam is US Food and Drug Administration-approved to treat insomnia (trouble with sleeping). It is typically taken short-term (approximately 7-10 days). It is a benzodiazepine, which are a class of medicines that are central nervous system depressants and slow down the nervous system. It is extensively metabolized via conjugation and N-demethylation to oxazepam. Its elimination half-life ranges from 3.5 to 18.4 hours.

Reference Values

Therapeutic concentrations

Temazepam: 600-1100 ng/mL at 1 hour

 

Cutoff concentrations by liquid chromatography tandem mass spectrometry:

Temazepam: 10 ng/mL

Oxazepam: 10 ng/mL

Day(s) Performed

Monday, Wednesday

Report Available

3 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80299