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Test Code SEMA3 Semaphorin 3B (SEMA3B) Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required, order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Specimen Type: Tissue

Supplies: Immunostain Technical Only Envelope (T693)

Container/Tube: Immunostain Technical Only Envelope

Preferred:

-Formalin-fixed, paraffin-embedded tissue block

OR

-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: None


Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen.

-Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763)

-Renal Diagnostics Test Request (T830)

Useful For

Aiding in the diagnosis of semaphorin 3B-associated membranous nephropathy

Method Name

Immunohistochemistry (IHC)

Reporting Name

SEMA3B IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides
Snowcoat slides		 Reject

Clinical Information

Primary membranous nephropathy (MN) is an autoimmune disease of the kidney where antibodies target an antigen in the glomerular basement membrane resulting in kidney damage or failure. In up to 75% of primary membranous nephropathy cases PLA2R (phospholipase A2 receptor) and THSD7A (thrombospondin type-1 domain-containing 7A) are the target antigens. SEMA3B (semaphorin 3B)-associated MN is a very rare disease that accounts for only 1% to 3% of all MN specimens. SEMA3B-associated MN is observed mostly in pediatric patients and young adults.

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC