Test Code PLABN Platelet Antibody Screen, Serum
Useful For
Detecting alloantibodies to epitopes on platelet glycoproteins IIb/IIIa, Ib/Ix, Ia/IIa, IV and class I human leukocyte antigens to evaluate cases of immune mediated refractoriness to platelet transfusions, posttransfusion purpura, or neonatal alloimmune thrombocytopenia
Special Instructions
Method Name
Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Platelet Ab Screen, SSpecimen Type
Serum RedOrdering Guidance
For neonate testing, consider sending a maternal specimen instead of a neonate specimen as unbound platelet antibodies may not be detected in the neonate serum.
This test is not recommended for the diagnosis of immune thrombocytopenia or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.
Necessary Information
If ordering electronically, answer all prompt questions for timely result reporting:
1. Reason for request is required for result interpretation.
a. Use provided diagnosis options if appropriate. If specific diagnosis is unknown, select the generic answer of alloimmune thrombocytopenia.
b. Record only the diagnosis pertaining to this test.
c. Record diagnosis description instead of code.
2. Indicate if patient has had intravenous immunoglobulin (IVIg) therapy in the last month: Yes or No
Specimen Required
Patient Preparation: Do not collect within 72 hours of a platelet transfusion. Transfused platelets will interfere with this assay.
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum Red | Frozen (preferred) | 365 days | |
Refrigerated | 48 hours |
Reject Due To
Gross hemolysis | Reject |
Clinical Information
Platelet antibodies may be allo- or autoantibodies and may be directed to a wide range of antigenic "targets" carried on platelet cytoplasmic membranes. Serum platelet antibody test is optimized to identify the presence of platelet alloantibodies in the patient.
Platelet alloantibodies are involved in several clinical situations such as:
-Immune mediated refractoriness to platelet transfusions usually due to antibodies to class I human leukocyte antigens and sometimes to antibodies specific to platelet antigens.
-Neonatal alloimmune thrombocytopenia
-Posttransfusion purpura, which are usually associated with platelet-specific antibodies
Reference Values
Not applicable
Day(s) Performed
Monday through Friday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86022