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HelpTest Code LLTOT Leukemia and Lymphoma Immunophenotyping, Technical Only, Tissue
Ordering Guidance
This test is available to clients through MayoAccess or MayoLink.
This test is not intended for product of conception (POC) specimens. For POC specimens see CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth.
Order LLTOF / Leukemia and Lymphoma Phenotyping, Technical Only, Varies if the specimen is a fresh (less than 4 days post-collection), unfixed, non-embedded bone marrow core biopsy, bone or bone lesion. This is an equivalent source for bone marrow aspirate only in the event of a dry tap during the bone marrow harvesting procedure. Indicate "dry tap" in performing lab notes or paperwork when submitting this specimen type.
Shipping Instructions
Specimen must arrive within 4 days of collection.
Necessary Information
The following information is required:
1. Reason for testing
2. Tissue type
3. Tissue location
4. Surgical pathology case number
Specimen Required
Submit 1 of the following specimens:
Preferred
Specimen Type: Tissue
Supplies: Hank's Solution (T132)
Container/Tube: Sterile container with 15 mL of tissue culture medium (eg, Hank's balanced salt solution, RPMI, or equivalent)
Specimen Volume: 5 mm(3) or larger biopsy
Collection Instructions:
1. Place tissue into a sterile container with 15 mL of tissue culture medium (eg, Hank's balanced salt solution, RPMI, or equivalent).
2. Send intact specimen (do not mince).
3. Specimen cannot be fixed.
Specimen Stability Information: Ambient 4 days/Refrigerated 4 days
Acceptable
Specimen Type: Fine needle aspirate (FNA)
Supplies: Hank's Solution (T132)
Container/Tube: Sterile container with 15 mL of tissue culture medium (eg, Hank's balanced salt solution, RPMI, or equivalent)
Specimen Volume: Entire collection
Collection Instructions:
1. Collect FNA and transfer entire collection into a sterile container with 15 mL of tissue culture medium (eg, Hank's balanced salt solution, RPMI, or equivalent).
2. Send intact specimen (do not mince).
3. Specimen cannot be fixed.
Specimen Stability Information: Ambient 4 days/Refrigerated 4 days
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Evaluation of tissues for potential involvement by:
-Chronic lymphoproliferative disorders
-Malignant lymphomas
-Acute lymphoblastic leukemia
-Acute myelogenous leukemia
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FCINT | Flow Cytometry Interp, 2-8 Markers | No, (Bill Only) | No |
| FCIMS | Flow Cytometry Interp, 9-15 Markers | No, (Bill Only) | No |
| FCINS | Flow Cytometry Interp,16 or greater | No, (Bill Only) | No |
Special Instructions
Method Name
Immunophenotyping
Reporting Name
Leukemia/Lymphoma; Tech Only TissueSpecimen Type
TissueSpecimen Minimum Volume
Tissue: 1 mm(3); Fine needle aspirate: See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Tissue | Refrigerated (preferred) | |
| Ambient | ||
Reject Due To
| Fixed, paraffin-embedded, or minced tissue | Reject |
| Dry tissue without transport medium | Reject |
Clinical Information
Cellular immunophenotyping, characterizing cells by using antibodies directed against cell surface markers, is generally regarded as a fundamental element in establishing a diagnosis of tissue involvement by hematolymphoid malignancies when used in conjunction with morphologic assessment. It is also an essential component in subclassification of hematolymphoid malignancies when present.
This is a technical only test and does not include interpretation. At any point, clients may request to have a Mayo Clinic hematopathologist provide an interpretation at an additional charge.
Reference Values
Not applicable
Day(s) Performed
Monday through Sunday
Report Available
1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker x 1
88185-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each)
Additional CPTs may be added if consultative help is needed with the case, or algorithm dictates Mayo consultant involvement.
88187-Flow Cytometry Interpretation, 2 to 8 Markers (if appropriate)
88188-Flow Cytometry Interpretation, 9 to 15 Markers (if appropriate)
88189-Flow Cytometry Interpretation, 16 or More Markers (if appropriate)