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Test Code HG34W Histone 3.3 G34W (H3F3A G34W) Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

Mayo Clinic Laboratories has multiple histone immunostains available. See table for ordering guidance. 

Test ID

Published name

Indication

Mayo Clinic
slide label

HG34W

Histone 3.3 G34W (H3F3A G34W) Immunostain, Technical Component Only

Giant cell tumor of bone (GCTB)

H3 G34W

HK27M

Histone H3 K27M Mutant (H3 K27M) Immunostain, Technical Component Only

K27M mutant midline gliomas

H3 K27M

HISME

Histone H3 Trimethyl K27 Immunostain, Technical Component Only

MPNST and K27M mutant midline gliomas

H3 K27me3

HK36M

Histone H3 K36M Mutant (H3F3 K36M) Immunostain, Technical Component Only

Chondroblastoma

H3 K36M


Shipping Instructions


Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Specimen Type: Tissue

Supplies: Immunostain Technical Only Envelope (T693)

Container/Tube: Immunostain Technical Only Envelope

Preferred:

-Formalin-fixed, paraffin-embedded tissue block

OR

-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: None


Useful For

Aiding in the diagnosis of giant cell tumor of bone

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

Histone H3 G34W IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides
Snowcoat slides		 Reject

Clinical Information

H3F3A is a member of the histone H3 family and is located on chromosome 1. Histone H3 G34W is expressed in the nuclei of the mononuclear cell population of H3F3A altered giant cell tumor of bone (GCTB).

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, Primary

88341-TC, If additional IHC

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.