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HelpTest Code HBRM SWI/SNF Chromatin Remodeling Complex Subunit ATPase 2 (SMARCA2 or BRM) Immunostain, Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Specimen Type: Tissue
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Submit:
Formalin-fixed, paraffin-embedded tissue block
OR
2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Forms
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Useful For
Identifying and further subtyping SWI/SNF complex subunit loss associated with malignant rhabdoid tumors, epithelioid sarcomas, BRG1 (SMARCA4) deficient tumors, and other poorly differentiated malignancies
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Method Name
Immunohistochemistry (IHC)
Reporting Name
BRM (SMARCA2) IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| TECHONLY | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Wet/frozen tissue | Reject |
| Cytology smears | Reject |
| Nonformalin fixed tissue | Reject |
| Nonparaffin embedded tissue | Reject |
| Noncharged slides | Reject |
| ProbeOn slides | Reject |
| Snowcoat slides | Reject |
Clinical Information
BRM is one of two mutually exclusive catalytic ATPase subunits of the SWI/SNF complex. The loss of at least one SWI/SNF subunit has been associated with undifferentiated carcinomas and/or advanced tumorigenesis. The homogenous and heterogenous loss of BRM can occur independently or concordantly with other SWI/SNF subunits.
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, Primary
88341-TC, If additional IHC