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HelpTest Code AFOLR Folate Receptor Alpha (FOLR1), Semi-Quantitative Immunohistochemistry, Manual
Ordering Guidance
This test may be utilized for all types of ovarian cancers.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.
Necessary Information
A pathology/diagnostic report and a brief history are required.
Specimen Required
Specimen Type: Tissue
Source: Ovarian tumor
Supplies: Pathology Packaging Kit (T554)
Collection Instructions: Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, "positively charged" slides with 4-microns formalin-fixed, paraffin-embedded tissue
Additional Information: One slide will be stained with hematoxylin and eosin and returned.
Useful For
Diagnosis of epithelial ovarian cancer that may be eligible for treatment with an anti-folate receptor 1 protein antibody
Method Name
Immunohistochemistry (IHC)
Reporting Name
FOLR1, SemiQuant IHC, ManualSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Special | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Wet/frozen tissue Decalcified paraffin embedded tissue Cytology smears Non-formalin fixed tissue including alcohol-formalin-acetic acid (AFA), 95% ethanol, PREFER fixatives or Zinc formalin Nonparaffin embedded tissue Noncharged slides ProbeOn slides |
Reject |
Clinical Information
Folate receptor 1 protein (FOLR1), also known as folate receptor alpha (FRa), is expressed in approximately 90% of ovarian carcinomas and serves as a predictive biomarker for FOLR1-targeted therapy for epithelial ovarian cancer. FOLR1 is a member of the folate receptor family that is reported to be highly expressed in various tumors of epithelial origin but has restricted expression in normal epithelial cells. Positivity for FOLR1 is expressed on greater or equal to 75% of viable tumor cells with moderate and/or strong membrane staining, while less than 75% of viable tumor cells with moderate and/or strong membrane staining is considered negative for FOLR1.
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88360
Forms
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.