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HelpTest Code STICK Tick-Borne Antibodies, Modified 2-Tier, ELISA, Serum
Ordering Guidance
During the acute phase of an Anaplasma phagocytophilum or Ehrlichia chaffeensis infection, serologic tests are often nonreactive, polymerase chain reaction (PCR) testing is available to aid in the diagnosis of these cases; see EHRL / Ehrlichia/Anaplasma, Molecular Detection, PCR, Blood.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.35 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluation of the most common tick-borne diseases found in the United States, including Lyme disease, human monocytic and granulocytic ehrlichiosis, and babesiosis using the modified 2-tier testing algorithm approach
Evaluation of patients with a history of, or suspected, tick exposure who are presenting with fever, myalgia, headache, nausea, and other nonspecific symptoms
Sero-epidemiological surveys of the prevalence of the infection in certain populations
Diagnosis of Lyme disease
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ANAP | Anaplasma phagocytophilum Ab, IgG,S | Yes | Yes |
| EHRC | Ehrlichia Chaffeensis (HME) Ab, IgG | Yes | Yes |
| BABG | Babesia microti IgG Ab, S | Yes | Yes |
| SLYME | Lyme Ab Modified 2-Tier w/Reflex, S | Yes | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TLYME | Lyme IgM/IgG, WCS, EIA, S | Yes | No |
Special Instructions
Method Name
ANAP, EHRC, BABG: Immunofluorescence Assay (IFA)
SLYME: Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Tick-Borne Abs w/ Lyme MTTTA, SSpecimen Type
SerumSpecimen Minimum Volume
1.1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 10 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-inactivated specimen | Reject |
Clinical Information
In North America, ticks are the primary vectors of infectious diseases.(1) Worldwide, ticks rank second only to mosquitoes in disease transmission. In the United States, tick-borne diseases include Lyme disease, Rocky Mountain spotted fever, human monocytic and granulocytic ehrlichiosis, babesiosis, tularemia, relapsing fever, and Colorado tick fever.
Symptoms of the various tick-vectored diseases range from mild to life-threatening and significantly overlap. Early symptoms, which include fever, aches, and malaise, do not aid in distinguishing the various diseases. Because early treatment can minimize or eliminate the risk of severe disease, early detection is essential, yet patients may not have developed distinctive symptoms to help in the differential diagnosis. A tick-borne panel can assist in identifying the pathogen, allowing treatment to be initiated.
For information on the specific diseases, see the individual test IDs.
Reference Values
Anaplasma phagocytophilum ANTIBODY, IgG
<1:64
Reference values apply to all ages.
Ehrlichia chaffeensis (HME) ANTIBODY, IgG
<1:64
Reference values apply to all ages.
Babesia microti IgG ANTIBODIES
<1:64
Reference values apply to all ages.
LYME ANTIBODY
Negative
Reference values apply to all ages.
Day(s) Performed
Monday through Friday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
See Individual Test IDsCPT Code Information
86618
86666 x 2
86753
86617 x 2 (if appropriate)
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.