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HelpTest Code MSMN Membranous Nephropathy Target Antigen Identification, Mass Spectrometry, Tissue
Ordering Guidance
This test should be performed on cases with a confirmed diagnosis of membranous nephropathy. Ideally, these cases should already be confirmed as phospholipase A2 receptor (PLA2R) negative, based on PLA2R immunohistochemistry (IHC) or immunofluorescence (IF) testing, not PLA2R serology testing.
If PLA2R IHC or IF testing has not been performed, order test PLAIF / Phospholipase A2 Receptor (PLA2R), Renal Biopsy and submit the additional required specimen.
Additional Testing Requirements
If phospholipase A2 receptor testing using immunohistochemistry or immunofluorescence (IF) has not previously been performed, PLAIF / Phospholipase A2 Receptor (PLA2R), Renal Biopsy must also be ordered and will be charged separately. An additional frozen IF block or frozen unstained slides will be required.
Necessary Information
The following information is required. Testing will not proceed without this information.
Note: Images can be submitted for the request via Mayo Clinic ImageShare (preferred) or external storage device. Be aware that ImageShare does not support the upload of Pathology whole slide images.
1. Preliminary pathology report and clinical history
2. Electron microscopy (EM) images
3. Immunofluorescence (IF) slides/images if EM images are not available.
Specimen Required
Specimen Type: Kidney tissue
Supplies: Pathology Packaging Kit (T554)
Container/Tube: Formalin-fixed, paraffin-embedded tissue block
Additional Information: Do not send fixed tissue slides. Testing can only be done on paraffin-embedded tissue blocks.
Useful For
Identification of antigen in membranous nephropathy
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MSMNT | Mass Spectrometry, MN TC | No, (Bill Only) | No |
| MNLCP | Microdissection, Laser Capture, MN | No, (Bill Only) | No |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
MN Target Antigen ID, LC MS, TsSpecimen Type
SpecialSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Special | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Fixed tissue slides Wet/frozen tissue Cytological smears Non-formalin fixed tissue Nonparaffin embedded tissue |
Reject |
Clinical Information
Membranous nephropathy (MN) is an autoimmune disease and a common cause of nephrotic syndrome in adults. MN results from glomerular accumulation of antigen-antibody complexes along the subepithelial region of the glomerular basement membranes. A series of novel antigens have recently been identified, and many of these antigen-associated MN have distinct clinical and pathologic findings as well as outcomes. This assay is intended to identify the antigens associated with MN using laser microdissection of MN glomeruli followed by mass spectrometry. The panel of MN antigens includes CNTN1, EXT1, EXT2, FAT1, HTRA1, NCAM1, NDNF, NELL1, PCDH7, PCSK6, PLA2R, SEMA3B, and THSD7A.
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
7 to 15 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
88380
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.