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Test Code MPQU Monoclonal Protein Quantitation, 24 Hour, Urine


Shipping Instructions


Refrigerate specimen during collection and send refrigerated.



Necessary Information


24-Hour volume (in milliliters) is required.



Specimen Required


Supplies: Urine Container, 60 mL (T313)

Submission Container/Tube: Plastic, 60-mL urine bottle

Specimen Volume: 50 mL

Collection Instructions:

1. Collect urine for 24 hours.

2. Aliquot between 30 mL and 50 mL urine into plastic, 60-mL urine bottle.

Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.


Useful For

Monitoring patients with monoclonal gammopathies using 24-hour urine collections

Profile Information

Test ID Reporting Name Available Separately Always Performed
PTU3 Protein, Total, 24 HR, U Yes, (Order PTU) Yes
PEU Protein Electrophoresis, 24 Hr, U No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MPTU M-protein Mass-Fix, 24 HR, U No No

Method Name

PTU3: Turbidimetry

PEU: Agarose Gel Electrophoresis

MPTU: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

Reporting Name

M-protein Quantitation, 24 HR, U

Specimen Type

Urine

Specimen Minimum Volume

30 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 14 days
  Frozen  5 days
  Ambient  24 hours

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability

Clinical Information

Urine proteins can be grouped into 5 fractions by protein electrophoresis:

-Albumin

-Alpha-1

-Alpha-2

-Beta-globulin

-Gamma-globulin

One or more quantifiable monoclonal proteins may be present and reported as M spike

 

The urine total protein concentration, the electrophoretic pattern, and the presence of a monoclonal immunoglobulin light chain may be characteristic of monoclonal gammopathies such as multiple myeloma, primary systemic amyloidosis, and light chain deposition disease.

 

The following algorithms are available:

-Amyloidosis: Laboratory Approach to the Diagnosis

-Multiple Myeloma: Laboratory Screening

Reference Values

PROTEIN, TOTAL

<229 mg/24 hours

 

Reference values have not been established for patients who are younger than 18 years of age.

 

ELECTROPHORESIS, PROTEIN

The following fractions, if present, will be reported as mg/24 hours:

Albumin

Alpha-1-globulin

Alpha-2-globulin

Beta-globulin

Gamma-globulin

 

MASS-FIX M-PROTEIN ISOTYPE

M-protein Isotype MS:

No monoclonal protein detected

 

Flag M-protein Isotype MS:

Negative

Day(s) Performed

Monday through Friday

Report Available

4 to 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84156

84166

0077U (if appropriate)