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Test Code CATTR Cold Agglutinin Titer, Serum


Ordering Guidance


The cold agglutinin test is not specific for Mycoplasma pneumoniae and is not recommended to diagnose M pneumoniae infections. To diagnose M pneumoniae infections, order MPRP / Mycoplasmoides pneumoniae, Molecular Detection, PCR, Varies. For screening recent or past exposure to M pneumoniae, order MYCO / Mycoplasma pneumoniae Antibodies, IgG and IgM, Serum.



Specimen Required


Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 4 mL

Pediatric Volume: 1 mL

Collections Instructions:

1. Use a warm pack to keep specimen at 37° C prior to and after collecting.

2. Allow specimen to clot at 37° C.

3. Centrifuge at 37° C and aliquot serum into plastic vial immediately after blood clots or within one hour of collection.

4. Do not refrigerate prior to separation of serum from red cells.


Useful For

Detection of cold agglutinins in patients with suspected cold agglutinin disease

 

This test is not recommended to diagnose Mycoplasma pneumoniae infections.

Method Name

Titration/Red Cell Agglutination

Reporting Name

Cold Agglutinin Titer

Specimen Type

Serum Red

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 7 days
  Frozen  7 days
  Ambient  72 hours

Reject Due To

Gross hemolysis OK
Gross icterus OK

Clinical Information

The cold agglutinin titer test is to be used as a tool in the evaluation of suspected cold agglutinin syndrome. In this syndrome, cold agglutinins, usually IgM with anti-I specificity, attach to the patient's erythrocytes causing a variety of symptoms. Symptoms may include chronic anemia due to premature removal of the sensitized erythrocytes from circulation by hemolysis, to acrocyanosis of the ears, fingers, or toes due to local blood stasis in the skin capillaries.

Reference Values

Titer results:

>64: Elevated

>1000: May be indicative of hemolytic anemia

Day(s) Performed

Monday through Friday, Sunday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86157

Forms

If not ordering electronically, complete, print, and send an Benign Hematology Test Request (T755) with the specimen.