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HelpTest Code B6PA Pyridoxic Acid, Plasma
Method Name
Only orderable as part of a profile. For more information see B6PRO / Vitamin B6 Profile (Pyridoxal 5-Phosphate and Pyridoxic Acid), Plasma.
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Pyridoxic Acid (PA), PSpecimen Type
Plasma HeparinShipping Instructions
Ship specimen in amber vial to protect from light.
Specimen Required
Only orderable as part of a profile. For more information see B6PRO / Vitamin B6 Profile (Pyridoxal 5-Phosphate and Pyridoxic Acid), Plasma.
Patient Preparation:
1. Patient should fast overnight (12-14 hours); infants-should have specimen collected before next feeding. Water can be taken as needed.
2. For 24 hours before specimen collection, patient must not take multivitamins or vitamin supplements.
Supplies: Amber Frosted Tube, 5 mL (T915)
Collection Container/Tube: Green top (sodium or lithium heparin) or plasma gel separator (PST)
Submission Container/Tube: Amber vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge at 4° C within 2 hours of collection.
2. Aliquot all plasma into amber vial and freeze immediately.
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Heparin | Frozen | 29 days | LIGHT PROTECTED |
Reference Values
Only orderable as part of a profile. For more information see B6PRO / Vitamin B6 Profile (Pyridoxal 5-Phosphate and Pyridoxic Acid), Plasma.
3-30 mcg/L
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
Useful For
Determining the concentration of pyridoxic acid in the assessment of vitamin B6 status
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Clinical Information
Vitamin B6 is a complex of 6 vitamers: pyridoxal, pyridoxol, pyridoxamine, and their 5'-phosphate esters. Due to its role as a cofactor in many enzymatic reactions, pyridoxal 5-phosphate (PLP) has been determined to be the biologically active form of vitamin B6.
Vitamin B6 deficiency is a potential cause of burning mouth syndrome and a possible potentiating factor for carpal tunnel and tarsal tunnel syndromes. Persons who present with chronic, progressive nerve compression disorders may be deficient in vitamin B6 and should be evaluated. Vitamin B6 deficiency is associated with symptoms of scaling of the skin, severe gingivitis, irritability, weakness, depression, dizziness, peripheral neuropathy, and seizures. In the pediatric population, deficiencies have been characterized by diarrhea, anemia, and seizures.
Markedly elevated PLP in conjunction with low levels of pyridoxic acid are observed in cases of hypophosphatasia, a disorder characterized by low levels of alkaline phosphatase and a range of skeletal abnormalities.
Day(s) Performed
Monday through Thursday, Saturday, Sunday