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Test Code TBSP Mycobacterium tuberculosis Complex Species Identification, PCR, Varies

Infectious


Ordering Guidance


This test should be used to identify the species within the Mycobacterium tuberculosis complex from a known M tuberculosis complex isolate.

 

For identification of M tuberculosis complex from isolate growth, order CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies.

 

For rapid identification of M tuberculosis complex directly from a specimen, order MTBRP / Mycobacterium tuberculosis Complex, Molecular Detection, PCR, Varies or MTBXS / Mycobacterium tuberculosis Complex, Molecular Detection and Rifampin Resistance, PCR, Sputum.



Shipping Instructions


1. For shipping information see Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.



Necessary Information


Specimen source and suspected organism identification are required.



Specimen Required


Specimen Type: Mycobacterium tuberculosis complex isolate growing in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube: Growth on solid slant media, eg, Middlebrook 7H10, 7H11 and Lowenstein Jensen; growth in broth medium, eg, Mycobacteria Growth Indicator Tube, 7H9 broth BACT/ALERT MP or VersaTREK

Specimen Volume: Isolate with visible growth on solid media; if broth is sent, 3 mL or more of broth culture

Collection Instructions:

1. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.

Additional Information: If subculture to Middlebrook agar medium is needed to ensure purity, turnaround time for results may be delayed.


Useful For

Determining the species of a Mycobacterium tuberculosis complex culture isolate

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

M tuberculosis species ID, PCR

Specimen Type

Varies

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

Agar plate
Mixed culture		 Reject

Clinical Information

This assay provides a species-level identification of microbiologic culture isolates previously identified to be a member of the Mycobacterium tuberculosis complex. Species level identification can be important for patient care or for epidemiologic investigations. For example, the species-level identification of Mycobacterium bovis bacillus Calmette-Guerin (BCG) can assist with identification of disseminated infections following use of the vaccine as an adjuvant during chemotherapy.

Reference Values

Not applicable

Day(s) Performed

Monday through Friday

Report Available

7 to 14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87150