Test Code FFTST Testosterone, Free and Weakly Bound, With Total Testosterone, LC/MS-MS
Specimen Required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions:
1. Draw blood in a plain red-top tube(s).
2. Within one hour of collection, centrifuge and aliquot 2 mL serum into a plastic vial.
3. Ship refrigerated.
Method Name
Ammonium sulfate precipitation; liquid chromatography/tandem mass spectrometry (LC/MS-MS)
Reporting Name
Testosterone,F/WklyBd+T LC/MSSpecimen Type
SerumSpecimen Minimum Volume
1.4 mL (does not allow for repeat testing)
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 7 days | |
Ambient | 7 days |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
Males:
Age: |
Range (ng/dL): |
Premature Infants (26 to 28 weeks) Day 4 |
59.0-125.0 |
Premature (31-35 weeks) Day 4 |
37.0-198.0 |
Newborns |
75.0-400.0 |
Prepubertal (1 to 10 years) |
<2.5-10.0 |
1 to 7 Months
Levels decrease rapidly the first week to 20.0-50.0 ng/dL, then increase to 60.0-400.0 ng/dL (mean=190.0) between 20-60 days. Levels then decline to prepubertal range of <2.5-10.0 by seven months.
Females:
Age: |
Range (ng/dL): |
Premature Infants (26 to 28 weeks) Day 4 |
5.0-16.0 |
Premature (31-35 weeks) Day 4 |
5.0-22.0 |
Newborns |
20.0-64.0 |
Prepubertal (1 to 9 years) |
<2.5-10.0 |
1 to 7 Months
Levels decrease during the first month to <10.0 ng/dL and remain there until puberty.
Puberty:
Tanner Stage (Males) |
Age (Years) |
Range (ng/dL) |
Mean (ng/dL) |
1 |
<9.8 |
<2.5-10.0 |
4.9 |
2 |
9.8-14.5 |
18.0-150.0 |
42.0 |
3 |
10.7-15.4 |
100.0-320.0 |
190.0 |
4 |
11.8-16.2 |
200.0-620.0 |
372.0 |
5 |
12.8-17.3 |
350.0-970.0 |
546.0 |
Tanner Stage (Females) |
Age (Years) |
Range (ng/dL) |
Mean (ng/dL) |
1 |
<9.2 |
<2.5-10.0 |
4.9 |
2 |
9.2-13.7 |
7.0-28.0 |
18.0 |
3 |
10.0-14.4 |
15.0-35.0 |
25.0 |
4 |
10.7-15.6 |
13.0-32.0 |
22.0 |
5 |
11.8-18.6 |
20.0-38.0 |
28.0 |
Adults:
≥18 Years |
Range (ng/dL) |
Males |
350.0-1030.0 |
Females |
|
Premenopausal |
10.0-55.0 |
Postmenopausal |
7.0-40.0 |
Testosterone % Free+Weakly Bound:
Female: 3.0-18.0%
Male: 9.0-46.0%
Testosterone, F+W Bound:
Female: 0.0-9.5 ng/dL
Male: 40.0-250.0 ng/dL
Day(s) Performed
Daily
Report Available
6 to 10 daysPerforming Laboratory
LabCorp BurlingtonTest Classification
This test was developed, and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.CPT Code Information
84410