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Test Code EOSU1 Eosinophils, Random, Urine


Specimen Required


Container/Tube: Unstained cytospin slide

Specimen Volume: 1 slide

Collection Instructions:

1. Collect a random urine specimen.

2. Centrifuge 10 mL of a random urine collection.

3. Pour off supernatant. Assess urine sediment for presence of white blood cells (WBC). If no WBC are seen, the eosinophil test cannot be performed. If WBC are present, prepare slides using the centrifuged sediment.

4. The unstained slide should be prepared within 2 hours of collection. Only an unstained slide will be accepted. Any other specimen, including the raw urine specimen, will be rejected.

5. Centrifuge urine in cytospin centrifuge at speed of 750 rpm for 5 minutes.

6. Slides need to be labeled with patient name and date of birth

7. Guidelines for slide preparation. Use the sediment to prepare slides as follows:

a. 150 mcL of sample for 1 to 3 WBC/high power field (hpf)

b. 100 mcL of sample for moderate to normal sediment (<50 cell/hpf)

c. 50 mcL of sample for heavier sediment (>50 cells/hpf)

d. 25 mcL for packed fields of sediment


Useful For

Investigation of possible acute interstitial nephritis

Method Name

Hansel Stain

Reporting Name

Eosinophils, U

Specimen Type

Urine

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Ambient (preferred) 14 days SLIDE
  Frozen  14 days SLIDE
  Refrigerated  14 days SLIDE

Reject Due To

Stained slides
Urine aliquots		 Reject

Clinical Information

Eosinophils are white blood cells that normally do not appear in urine. The presence of eosinophils in the urine is seen in acute interstitial nephritis, which is caused by an allergic reaction, typically to drugs.

Reference Values

0%

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

85999

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 day

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.