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HelpTest Code BCATN Beta-Catenin Immunostain, Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Specimen Type: Tissue
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
-Formalin-fixed, paraffin-embedded tissue block
OR
-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Acceptable: None
Forms
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Useful For
Identification of aberrant nuclear staining pattern observed in some tumors
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Method Name
Immunohistochemistry (IHC)
Reporting Name
B-Catenin IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| TECHONLY | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides Snowcoat slides |
Reject |
Clinical Information
Beta-catenin is involved in cellular cohesion through binding to the cytoplasmic tail of epithelial cadherin and in intracellular signaling as a component of the Wnt pathway. In normal cells, beta-catenin levels are regulated by the adenomatous polyposis coli (APC) protein, which promotes normal degradation of the protein. Alterations in either beta-catenin or APC can result in accumulation of the protein and abnormal localization in the nucleus. In the nucleus, beta-catenin acts as a cofactor in the upregulation of oncogenes including cyclin D1 and cmyc. In normal tissues, staining for beta-catenin is limited to the membrane. Aberrant nuclear staining can be used diagnostically in selected tumors of the soft tissue (fibromatoses, endometrial stromal sarcoma), pancreas, liver, and lung.
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, primary
88341-TC, if additional IHC
Specimen Minimum Volume
See Specimen Required